Coding

Part:BBa_K4817005:Design

Designed by: Yexi Liang   Group: iGEM23_SCU-China   (2023-10-10)


Ind


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Efficient expression of extracellular antimicrobial peptide Indolicidin expression elements induced by IPTG under the regulation of T7 promoter.The signal peptide OmpA fused at the N-terminus of Indolicidin cleaves and separates from each other at the C-terminus ASA sequence of OmpA during the process of assisting Indolicidin exocrine secretion.


Source

Bovine neutrophils

References